p2y 1 r drugs Search Results


p2y1r  (Novus Biologicals)
92
Novus Biologicals p2y1r
Mechanical ventilation increased alveolar free ATP and activated P2Y1 receptor. The mice were mechanically ventilated, and the low tidal volume and high tidal volume were set. The mice with spontaneous breathing were used as the control. A: ATP level in mouse BALF detected by luciferase assay, N = 6; B: PY1R expression pattern in lung tissues detected by Western blot, N = 6; C: ATP level in MLE-12 cells detected by luciferase assay; D: PY1R expression pattern in MLE-12 cells detected by Western blot. Cell experiments were conducted 3 times independently. ** p < 0.01. Data in panels A and B were analyzed using one-way ANOVA, followed by Tukey’s multiple comparisons test, and data in panels C and D were analyzed using t test. ATP, adenosine triphosphate; RLU, relative light unit; BALF, bronchoalveolar lavage fluid; LVT, low tidal volume; HVT, high tidal volume; <t>P2Y1R,</t> P2Y1 receptor; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.
P2y1r, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p2y+1+r+drugs/pmc08974168-70-4-12?v=Novus+Biologicals
Average 92 stars, based on 1 article reviews
p2y1r - by Bioz Stars, 2026-07
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Mechanical ventilation increased alveolar free ATP and activated P2Y1 receptor. The mice were mechanically ventilated, and the low tidal volume and high tidal volume were set. The mice with spontaneous breathing were used as the control. A: ATP level in mouse BALF detected by luciferase assay, N = 6; B: PY1R expression pattern in lung tissues detected by Western blot, N = 6; C: ATP level in MLE-12 cells detected by luciferase assay; D: PY1R expression pattern in MLE-12 cells detected by Western blot. Cell experiments were conducted 3 times independently. ** p < 0.01. Data in panels A and B were analyzed using one-way ANOVA, followed by Tukey’s multiple comparisons test, and data in panels C and D were analyzed using t test. ATP, adenosine triphosphate; RLU, relative light unit; BALF, bronchoalveolar lavage fluid; LVT, low tidal volume; HVT, high tidal volume; P2Y1R, P2Y1 receptor; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.

Journal: Bioengineered

Article Title: Mechanical ventilation induces lung and brain injury through ATP production, P2Y1 receptor activation and dopamine release

doi: 10.1080/21655979.2021.2022269

Figure Lengend Snippet: Mechanical ventilation increased alveolar free ATP and activated P2Y1 receptor. The mice were mechanically ventilated, and the low tidal volume and high tidal volume were set. The mice with spontaneous breathing were used as the control. A: ATP level in mouse BALF detected by luciferase assay, N = 6; B: PY1R expression pattern in lung tissues detected by Western blot, N = 6; C: ATP level in MLE-12 cells detected by luciferase assay; D: PY1R expression pattern in MLE-12 cells detected by Western blot. Cell experiments were conducted 3 times independently. ** p < 0.01. Data in panels A and B were analyzed using one-way ANOVA, followed by Tukey’s multiple comparisons test, and data in panels C and D were analyzed using t test. ATP, adenosine triphosphate; RLU, relative light unit; BALF, bronchoalveolar lavage fluid; LVT, low tidal volume; HVT, high tidal volume; P2Y1R, P2Y1 receptor; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.

Article Snippet: The primary antibodies included P2Y1R (NBP1-69,246, at a dilution ratio of 1:1000, Novus Biologicals, Littleton, CO, USA), dysbindin-1 (PA5-27,371, at a dilution ratio of 1:1000, Thermo Fisher Scientific), and GAPDH (ab8245, at a dilution ratio of 1:2000, Abcam).

Techniques: Control, Luciferase, Expressing, Western Blot

Activated P2Y1 receptor promoted DA release in mouse hippocampus. The mice were mechanically ventilated, and the low tidal volume and high tidal volume were set. The mice with spontaneous breathing were used as the control. Based on the high tidal volume, mice were performed with 330-min high positive-pressure ventilation. A: DA level in mouse hippocampus detected by ELISA, N = 6; B: dysbindin-1 level in mouse hippocampus detected by Western blot, N = 6; mouse hippocampal neuron cell line HT-22 were treated with P2Y1 receptor activator MRS2365 with cells treated with ACSF as controls, C: DA level in HT-22 cells detected by ELISA; D: levels of P2Y1R and dysbindin-1 in HT-22 cells detected by Western blot. Cell experiments were conducted 3 times independently. ** p < 0.01. Data were analyzed using one-way ANOVA, followed by Tukey’s multiple comparisons test. DA, dopamine; LVT, low tidal volume; HVT, high tidal volume; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; ACSF, artificial cerebral spinal fluid; P2Y1R, P2Y1 receptor.

Journal: Bioengineered

Article Title: Mechanical ventilation induces lung and brain injury through ATP production, P2Y1 receptor activation and dopamine release

doi: 10.1080/21655979.2021.2022269

Figure Lengend Snippet: Activated P2Y1 receptor promoted DA release in mouse hippocampus. The mice were mechanically ventilated, and the low tidal volume and high tidal volume were set. The mice with spontaneous breathing were used as the control. Based on the high tidal volume, mice were performed with 330-min high positive-pressure ventilation. A: DA level in mouse hippocampus detected by ELISA, N = 6; B: dysbindin-1 level in mouse hippocampus detected by Western blot, N = 6; mouse hippocampal neuron cell line HT-22 were treated with P2Y1 receptor activator MRS2365 with cells treated with ACSF as controls, C: DA level in HT-22 cells detected by ELISA; D: levels of P2Y1R and dysbindin-1 in HT-22 cells detected by Western blot. Cell experiments were conducted 3 times independently. ** p < 0.01. Data were analyzed using one-way ANOVA, followed by Tukey’s multiple comparisons test. DA, dopamine; LVT, low tidal volume; HVT, high tidal volume; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; ACSF, artificial cerebral spinal fluid; P2Y1R, P2Y1 receptor.

Article Snippet: The primary antibodies included P2Y1R (NBP1-69,246, at a dilution ratio of 1:1000, Novus Biologicals, Littleton, CO, USA), dysbindin-1 (PA5-27,371, at a dilution ratio of 1:1000, Thermo Fisher Scientific), and GAPDH (ab8245, at a dilution ratio of 1:2000, Abcam).

Techniques: Control, Enzyme-linked Immunosorbent Assay, Western Blot